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Study of selected mushroom toxins of Amanita muscaria.
Adámek, Michal ; Bosáková, Zuzana (advisor) ; Hložek, Tomáš (referee)
The presented bachelor's thesis was focused on the development of an HPLC-MS/MS method for the separation and eventual quantification of the main toxins of Amanita muscaria (fly agaric). After optimization of tandem mass detection conditions, MRM transitions were found for muscarine (174 → 57), ibotenic acid (159 → 113), muscimol (115 → 98) and cycloserine (103 → 75), which was used as an internal standard. Measurements were performed on Luna Omega 1.6 µm polar C18 column in RP-HPLC mode with an optimized mobile phase composition of 2:98 (v/v) acetonitrile/deionized water with the addition of 0.1% formic acid in both components and Ascentis Express RP- Amide in HILIC mode with an optimized mobile phase composed of 70:30 (v/v) methanol/deionized water with the addition of 0.5% formic acid in both components. Calibration curves with a range of 0.01 to 10 µg/ml for RP-HPLC and 0.01 to 25 µg/ml for HILIC were measured under optimized conditions. Urine and blood serum solutions of a patient with Amanita muscaria poisoning, including diluted versions of these solutions, were measured. Ibotenic acid and muscimol were found in the patient's urine, muscarine, ibotenic acid and muscimol in the blood serum. The RP-HPLC method gave narrower peaks with lower retentions and lower noise levels. The studied toxins...
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"Gentlemen, please, take of your hats": Methods of dating and interpretation of photographies from years 1850–1918 through historical mens fashion
Polách, Radek
„GENTLEMEN, PLEASE, TAKE OFF YOUR HATS“ – Methods of dating and interpretation of photographies from years 1850-1918 through historical mens fashion is compacted material for professional description of historical photographic material from noblemens collection funds based on basic knowledge of mens and partially child fashion, and related clothing accessories. In its entirety it brings a large amount of knowledge from history of the then mens fashion, which was after the downfall of the Austro-Hungarian empire overlooked or presented only in narrowly specialized sections, e.g. in the military section. A large part of this publication is based not only on the knowledge of description of mens clothing, but mainly of headwear. It is this clothing accessory that is pivotal to the observed era, and with a closer look, it can help accurately date and describe people captured on historical photographs. It is primarily meant for the specialized staff of memory institutions of Czech Republic, who deal with these given issues. At the same though, it can allow curators and owners of historical funds a way to describe collections of exhibits that are kept in their collection funds.
Fulltext:  PDF
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